Post Doctoral Fellow-MSH-12908-021

Department: Medicine/Nephrology

Physical work location: 1468 Madison Avenue, Annenberg 23-16, New York, NY 10029

Name PI or Supervisor: John Cijiang He

Web link to Lab: https://labs.icahn.mssm.edu/helab/meet-the-team/

Web link to Department: https://icahn.mssm.edu/about/departments-offices/medicine/nephrology 

Administrative Contact: Irene Beaulieu; email: Irene.Beaulieu@mssm.edu

Background and Description of Research Project: 

Diabetic kidney disease is the number one cause of kidney failure in the USA as well as in the world. Its underlined pathogenesis remains unclear and the treatment is limited. We have been using systems biology approach to study its pathogenesis in order to identify potential new drug targets to treat this disease. Recently, we identified TENM3 as a key molecule involving in glomerular podocyte injury in diabetic kidney disease. The current project will focus on the role of TENM3 in the pathogenesis of diabetic kidney disease. We will use in vitro assays and in vivo animal models to confirm the critical role of TENM3 in podocyte injury in diabetic kidney disease. 

Technical Duties (include any protocols):

• Perform in vitro cell cultures of glomerular podocytes and test whether overexpression or knockdown of TENM3 affects podocyte function. 
• We will generate mutant constructs of TENM3 to mimic human variants. We will perform qPCR and Western blot analysis of TENM3 expression. 
• We have generated transgenic mouse models with overexpression or knockdown of TENM3 in podocytes. We will test whether these mice develop more or less kidney injury.
• Techniques include cell culture, western blot, qPCR, genotyping of mice, mouse breading, kidney histology analysis, immunostaining, determination of urine albumin and renal function. 

Educational and other Requirements for the position: Postdoc should have medical training (MD equivalent) or basic science training and is familiar with kidney disease pathogenesis (Nephrology training). 

Experience Required: Candidate should have basic training in medical research and know how to perform basic bench work and animal studies.

Compensation Statement

The Mount Sinai Health System (MSHS) provides salary ranges that comply with the New York City Law on Salary Transparency in Job Advertisements. The salary range for the role is $74,692.00 - $80,000.00 Annually. Actual salaries depend on a variety of factors, including experience, education, and operational need. The salary range or contractual rate listed does not include bonuses/incentive, differential pay or other forms of compensation or benefits.

SPOC-UAW Local 4100 at Icahn School of Medicine (Post Docs), 036 - Renal MSH - ISM, Icahn School of Medicine